SARS CoV-2 HexaPro spike (bio-His)
(Plasmid #166856)

• Purpose: Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.
• Depositing Lab: Evelien Bunnik
• Publication: Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. doi: 10.1371/journal.pone.0261656. eCollection 2021.

Backbone

• Vector backbone: pαH
• Total vector size (bp): 8331
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS CoV-2 HexaPro full length spike protein
• Species: Severe acute respiratory syndrome-related coronavirus
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Tags / Fusion Proteins:
  • 8xHis (C terminal on insert)
  • Biotinylation site

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid modified and submitted by Sebastiaan Bol, Raphael Reyes, Katie Clarke and Evelien Bunnik. Expression was confirmed by SDS-PAGE and Western blot (anti-His and anti-biotin). Biotin ligase (BirA) and free biotin are required for biotinylation of the biotinylation site.