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Addgene

pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)
(Plasmid #166854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS424
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atg13[571–700]
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    F641E, I645E
  • Entrez Gene
    ATG13 (a.k.a. YPR185W, APG13)
  • Promoter pCu
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCu-His6-Atg13[571–700] F641E, I645E-Ss(424) was a gift from Daniel Klionsky (Addgene plasmid # 166854 ; http://n2t.net/addgene:166854 ; RRID:Addgene_166854)

  • For your References section:

    The carboxy terminus of yeast Atg13 binds phospholipid membrane via motifs that overlap with the Vac8-interacting domain. Gatica D, Damasio A, Pascual C, Klionsky DJ, Ragusa MJ, Popelka H. Autophagy. 2020 Jun;16(6):1007-1020. doi: 10.1080/15548627.2019.1648117. Epub 2019 Aug 2. 10.1080/15548627.2019.1648117 PubMed 31352862
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