pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)
(Plasmid
#166853)
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PurposeExpresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS424
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAtg13[571–700]
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SpeciesS. cerevisiae (budding yeast)
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MutationF641G, I645G
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Entrez GeneATG13 (a.k.a. YPR185W, APG13)
- Promoter pCu
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCu-His6-Atg13[571–700] F641G, I645G-Ss(424) was a gift from Daniel Klionsky (Addgene plasmid # 166853 ; http://n2t.net/addgene:166853 ; RRID:Addgene_166853) -
For your References section:
The carboxy terminus of yeast Atg13 binds phospholipid membrane via motifs that overlap with the Vac8-interacting domain. Gatica D, Damasio A, Pascual C, Klionsky DJ, Ragusa MJ, Popelka H. Autophagy. 2020 Jun;16(6):1007-1020. doi: 10.1080/15548627.2019.1648117. Epub 2019 Aug 2. 10.1080/15548627.2019.1648117 PubMed 31352862