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pRS416 ATG5-E141D-HA-ATG12-ATG16
(Plasmid #166851)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATG5 ATG12 ATG16
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ATG5 (a.k.a. YPL149W, APG5)
  • Entrez Gene
    ATG12 (a.k.a. YBR217W, APG12)
  • Entrez Gene
    ATG16 (a.k.a. YMR159C, APG15, APG16, CVT11, SAP18)
  • Tag / Fusion Protein
    • HA-ATG12 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS416 ATG5-E141D-HA-ATG12-ATG16 was a gift from Daniel Klionsky (Addgene plasmid # 166851 ; http://n2t.net/addgene:166851 ; RRID:Addgene_166851)

  • For your References section:

    Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay. Kim M, Sandford E, Gatica D, Qiu Y, Liu X, Zheng Y, Schulman BA, Xu J, Semple I, Ro SH, Kim B, Mavioglu RN, Tolun A, Jipa A, Takats S, Karpati M, Li JZ, Yapici Z, Juhasz G, Lee JH, Klionsky DJ, Burmeister M. Elife. 2016 Jan 26;5. pii: e12245. doi: 10.7554/eLife.12245. 10.7554/eLife.12245 PubMed 26812546
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