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pRS405 SEC66-VAC8-GFP
(Plasmid #166840)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS405
  • Total vector size (bp) 8453
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VAC8
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2544
  • Mutation
    SEC66 transmembrane domain
  • Entrez Gene
    VAC8 (a.k.a. YEL013W, YEB3)
  • Entrez Gene
    SEC66 (a.k.a. YBR171W, HSS1, KAR7, SEC71)
  • Promoter VAC8 promoter
  • Tags / Fusion Proteins
    • GFP (C terminal on insert)
    • SEC66 transmembrane domain (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearize with AflII

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS405 SEC66-VAC8-GFP was a gift from Daniel Klionsky (Addgene plasmid # 166840 ; http://n2t.net/addgene:166840 ; RRID:Addgene_166840)

  • For your References section:

    Vac8 determines phagophore assembly site vacuolar localization during nitrogen starvation-induced autophagy. Gatica D, Wen X, Cheong H, Klionsky DJ. Autophagy. 2020 Jun 8. doi: 10.1080/15548627.2020.1776474. 10.1080/15548627.2020.1776474 PubMed 32508216
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