pRS405 VAC8-GFP
(Plasmid
#166836)
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PurposeExpresses Vac8-GFP in yeasts.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166836 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS405
- Total vector size (bp) 8357
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVAC8
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2448
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Entrez GeneVAC8 (a.k.a. YEL013W, YEB3)
- Promoter VAC8 promoter
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize with AflII
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS405 VAC8-GFP was a gift from Daniel Klionsky (Addgene plasmid # 166836 ; http://n2t.net/addgene:166836 ; RRID:Addgene_166836) -
For your References section:
Vac8 determines phagophore assembly site vacuolar localization during nitrogen starvation-induced autophagy. Gatica D, Wen X, Cheong H, Klionsky DJ. Autophagy. 2020 Jun 8. doi: 10.1080/15548627.2020.1776474. 10.1080/15548627.2020.1776474 PubMed 32508216
