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pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized
(Plasmid #166834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 LIC 6LD
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLRP1
  • Alt name
    Codon-optimized NLRP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    NLRP1 (a.k.a. AIADK, CARD7, CIDED, CLR17.1, DEFCAP, DEFCAP-L/S, JRRP, MSPC, NAC, NALP1, PP1044, SLEV1, VAMAS1)
  • Promoter CMV
  • Tag / Fusion Protein
    • TEV-GFP-FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV F
  • 3′ sequencing primer GFP reverse (5'cagctcgaccaggatgggc3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized was a gift from Hao Wu (Addgene plasmid # 166834 ; http://n2t.net/addgene:166834 ; RRID:Addgene_166834)

  • For your References section:

    DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation. Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H. Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 10.1038/s41586-021-03350-4. 10.1038/s41586-021-03350-4 PubMed 33731932
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