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pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A
(Plasmid #166832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166832 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Vector type
    Gateway shuttling vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLRP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    NLRP1 (a.k.a. AIADK, CARD7, CIDED, CLR17.1, DEFCAP, DEFCAP-L/S, JRRP, MSPC, NAC, NALP1, PP1044, SLEV1, VAMAS1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A was a gift from Daniel Bachovchin (Addgene plasmid # 166832 ; http://n2t.net/addgene:166832 ; RRID:Addgene_166832)

  • For your References section:

    DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation. Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H. Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 10.1038/s41586-021-03350-4. 10.1038/s41586-021-03350-4 PubMed 33731932
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