Skip to main content
Addgene

dTAG NLRP1 P1214R
(Plasmid #166826)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLEX_305-N-dTAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLRP1
  • Species
    H. sapiens (human)
  • Mutation
    P1214R
  • Entrez Gene
    NLRP1 (a.k.a. AIADK, CARD7, CIDED, CLR17.1, DEFCAP, DEFCAP-L/S, JRRP, MSPC, NAC, NALP1, PP1044, SLEV1, VAMAS1)
  • Promoter hPGK

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N terminal dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dTAG NLRP1 P1214R was a gift from Daniel Bachovchin (Addgene plasmid # 166826 ; http://n2t.net/addgene:166826 ; RRID:Addgene_166826)

  • For your References section:

    DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation. Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H. Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 10.1038/s41586-021-03350-4. 10.1038/s41586-021-03350-4 PubMed 33731932
Related items: