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Addgene

pETDuet-LahT150
(Plasmid #166785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166785 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 5831
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LahT150
  • Species
    Lachnospiraceae bacterium C6A11
  • Insert Size (bp)
    447
  • GenBank ID
    HCA21450.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-LahT150 was a gift from Wilfred van der Donk (Addgene plasmid # 166785 ; http://n2t.net/addgene:166785 ; RRID:Addgene_166785)

  • For your References section:

    Insights into AMS/PCAT transporters from biochemical and structural characterization of a double Glycine motif protease. Bobeica SC, Dong SH, Huo L, Mazo N, McLaughlin MI, Jimenez-Oses G, Nair SK, van der Donk WA. Elife. 2019 Jan 14;8. pii: 42305. doi: 10.7554/eLife.42305. 10.7554/eLife.42305 PubMed 30638446
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