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pLV-mTurquoise2-IL-1beta-mNeonGreen
(Plasmid #166783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-eGFP
  • Backbone manufacturer
    Pantelis Tsoulfas (Addgene #36083)
  • Backbone size w/o insert (bp) 7435
  • Total vector size (bp) 8964
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    optimal growth in low salt LB (5gm NaCl)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mTurquoise2-IL-1b-mNeongreen
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2277
  • Entrez Gene
    IL1B (a.k.a. IL-1, IL1-BETA, IL1F2, IL1beta)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mTurquoise2 (N terminal on insert)
    • mNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV fwd
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-mTurquoise2-IL-1beta-mNeonGreen was a gift from Hao Wu (Addgene plasmid # 166783 ; http://n2t.net/addgene:166783 ; RRID:Addgene_166783)

  • For your References section:

    HDAC6 mediates an aggresome-like mechanism for NLRP3 and pyrin inflammasome activation. Magupalli VG, Negro R, Tian Y, Hauenstein AV, Di Caprio G, Skillern W, Deng Q, Orning P, Alam HB, Maliga Z, Sharif H, Hu JJ, Evavold CL, Kagan JC, Schmidt FI, Fitzgerald KA, Kirchhausen T, Li Y, Wu H. Science. 2020 Sep 18;369(6510). pii: 369/6510/eaas8995. doi: 10.1126/science.aas8995. 10.1126/science.aas8995 PubMed 32943500
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