Cdc42 T17N pCS2
(Plasmid
#16670)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16670 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2 (modified)
- Backbone size w/o insert (bp) 4000
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Vector typeVertebrate
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdc42 T17N
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Alt nameCdc42
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SpeciesH. sapiens (human)
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MutationT17N. Dominant negative.
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Entrez GeneCDC42 (a.k.a. CDC42Hs, G25K, TKS)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This cDNA encodes a dominant negative version of Cdc42, a Rho GTPase.
The vector is pCS2, but note the SP6 PROMOTER IS ABSENT in this construct. Thus use for DNA injection/transfection relying on the CMV promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cdc42 T17N pCS2 was a gift from De-Li Shi (Addgene plasmid # 16670 ; http://n2t.net/addgene:16670 ; RRID:Addgene_16670) -
For your References section:
Role of frizzled 7 in the regulation of convergent extension movements during gastrulation in Xenopus laevis. Djiane A, Riou J, Umbhauer M, Boucaut J, Shi D. Development. 2000 Jul . 127(14):3091-100. PubMed 10862746