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deltaVP1 + VP2C-GFP
(Plasmid #166676)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166676 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pILGFPB5A
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VP2C-GFP
  • Alt name
    VP2C-yEGFP
  • Alt name
    Murine polyomavirus VP2(251-301)-GFP
  • Species
    S. cerevisiae (budding yeast), Synthetic; Murine polyomavirus
  • Insert Size (bp)
    927
  • Promoter GAL10

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGGTAATGCCATGTAATATGATTATTAAAC
  • 3′ sequencing primer GCATTGGCACGGTGCAACAC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
  • Alt name
    MPyV deltaVP1
  • Species
    S. cerevisiae (budding yeast), Synthetic; Murine polyomavirus
  • Insert Size (bp)
    1143
  • Promoter GAL1

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACTTCTTATTCAAATGTCATAAAAGTATCAAC
  • 3′ sequencing primer CCAGCCTGCTTTTCTGTAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    deltaVP1 + VP2C-GFP was a gift from Claudia Vickers (Addgene plasmid # 166676 ; http://n2t.net/addgene:166676 ; RRID:Addgene_166676)

  • For your References section:

    Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in Yeast. Cheah LC, Stark T, Adamson LSR, Abidin RS, Lau YH, Sainsbury F, Vickers CE. ACS Synth Biol. 2021 Dec 17;10(12):3251-3263. doi: 10.1021/acssynbio.1c00045. Epub 2021 Sep 30. 10.1021/acssynbio.1c00045 PubMed 34591448
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