pGY577
(Plasmid #166663)

• Purpose: Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination
• Depositing Lab: Gaël Yvert
• Publication: Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi: 10.7554/eLife.61268.

Backbone

• Vector backbone: PGAE0 Self-Inactivating Vector
• Backbone size w/o insert (bp): 7208
• Vector type: Lentiviral, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LiCre
• Alt name: AsLOV2-CreE340AD341A
• Species: Synthetic; Avena sativa, Bacteriopage P4
• Insert Size (bp): 1497
• Mutation: Cre: E340A, D341A
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Age1 (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pGY577.gb
• A portion of this plasmid was derived from a plasmid made by: Vector pGAE0 originates from Mangeot et al. Nucleic Acids Research, 2004, Vol. 32, No. 12 e102

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a 24bp deletion that removes one of the SV40 NLS tags. This deletion is not known to affect plasmid function.