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Addgene

pGY466
(Plasmid #166661)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166661 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSH63
  • Backbone manufacturer
    Euroscarf
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LiCre
  • Alt name
    AsLOV2-CreE340AD341A
  • Species
    Synthetic; Avena sativa, Bacteriopage P2
  • Mutation
    Cre: E340A, D341A
  • Promoter MET17

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Vector pSH63 originates from Gueldener et al. Nucleic Acids Research, 2002, Vol. 30, No. 6 e23

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains 6 nucleotide discrepancies in the Met17 promoter sequence. These discrepancies are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGY466 was a gift from Gaël Yvert (Addgene plasmid # 166661 ; http://n2t.net/addgene:166661 ; RRID:Addgene_166661)

  • For your References section:

    A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch. Duplus-Bottin H, Spichty M, Triqueneaux G, Place C, Mangeot PE, Ohlmann T, Vittoz F, Yvert G. Elife. 2021 Feb 23;10. pii: 61268. doi: 10.7554/eLife.61268. 10.7554/eLife.61268 PubMed 33620312
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