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Addgene

pFOK
(Plasmid #166651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    R6K
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    JKe201 strain must be used for conjugation and other experimentation with this plasmid
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    I-sceI, sacB

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFOK was a gift from Dirk Bumann (Addgene plasmid # 166651 ; http://n2t.net/addgene:166651 ; RRID:Addgene_166651)

  • For your References section:

    Efficient dual-negative selection for bacterial genome editing. Cianfanelli FR, Cunrath O, Bumann D. BMC Microbiol. 2020 May 24;20(1):129. doi: 10.1186/s12866-020-01819-2. 10.1186/s12866-020-01819-2 PubMed 32448155
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