pGRG25
(Plasmid #16665)

• Depositing Lab: Nancy Craig
• Publication: McKenzie et al BMC Microbiol. 2006 . 6():39.

Backbone

• Vector backbone: pGRG25
• Backbone size w/o insert (bp): 12546
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha in LB + ampicillin, grow at less than 32 degrees C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mTn7::MCS

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AvrII, NotI, PacI, and XhoI (not destroyed)
• 3′ cloning site: AvrII, NotI, PacI, and XhoI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Supplemental Documents:
  • Transgene Insertion Protocol
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Grow at 32'C. Same as pGRG36 except for the MCS. See author's protocol for more information.

This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.