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Addgene

CAG-GFP Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: BamHI and BamHI+PmeI double digest.

We expected the following band sizes for this digest: 7.5kb for single digest and 0.7kb+6.8kb for the double. We see the expected pattern.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Ladder and a digestion of the plasmid
CAG-GFP with the following enzymes: KpnI-HF and NheI-HF