CAG-GFP
(Plasmid #16664)

• Depositing Lab: Fred Gage
• Publication: Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Backbone

• Vector backbone: CAG-GFP
• Backbone size w/o insert (bp): 6800
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 700

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI, SfiI, or AgeI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 24 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map for more information. CAG–GFP uses the backbone of pCLNCX v.2, and the expression of GFP is driven by the compound promoter CAG. This vector also contains the woodchuck hepatitis virus posttranscriptional element, which reportedly increases protein production by stabilizing the transcript.