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PurposeEncodes Flp-dependent tTA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametTA
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SpeciesSynthetic
- Promoter human synapsin 1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSYN1-fDIO-tTA was a gift from Martin Myers (Addgene plasmid # 166597 ; http://n2t.net/addgene:166597 ; RRID:Addgene_166597) -
For your References section:
tTARGIT AAVs mediate the sensitive and flexible manipulation of intersectional neuronal populations in mice. Sabatini PV, Wang J, Rupp AC, Affinati AH, Flak JN, Li C, Olson DP, Myers MG Jr. Elife. 2021 Mar 11;10. pii: 66835. doi: 10.7554/eLife.66835. 10.7554/eLife.66835 PubMed 33704065