t1-400
(Plasmid #166577)

• Purpose: For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
• Depositing Lab: Vesa Hytönen
• Publication: Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;117(51):32402-32412. doi: 10.1073/pnas.2014583117. Epub 2020 Dec 7.

Backbone

• Vector backbone: pTrcHis C
• Backbone manufacturer: Thermo Fisher Scientific, Carlsbad, CA
• Total vector size (bp): 5630
• Modifications to backbone: includes an N-terminal hexahistidine tag (MGGSHHHHHHGMASMTGGQQMGRDLYDDDD- KDRWIRPRA) before the insert.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21-Star (DE3), or other suitable strain for expression. 37C for 5h growing after induction. At lower temperature can be O/N. IPTG as the inducing agent
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tln1 Head (1-400)
• Alt name: talin-1
• Species: H. sapiens (human)
• Insert Size (bp): 1202
• Mutation: residues 1-400 only
• Entrez Gene: TLN1 (a.k.a. ILWEQ, TLN, talin-1)
• Tag / Fusion Protein:
  • His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry