LIP-WT
(Plasmid #16657)

• Depositing Lab: Bert Vogelstein
• Publication: Cheong et al Science. 2006 Nov 24. 314(5803):1308-11.

Backbone

• Vector backbone: pCR2.1 / T7-GFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Liposomase
• Species: Clostridium novyi-NT (anaerobic bacterium)
• Insert Size (bp): 1300
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The coding sequence of Liposomase, devoid of its N-terminal secretion signal, was PCR-amplified and cloned with an N-terminal 6xHis tag into the pCR2.1/T7-GFP vector.