pME-mKate2-P2A-TurboID-NS
(Plasmid
#166568)
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PurposeMultisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166568 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR 221
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Vector typemultisite gateway entry clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemKate2-P2A-TurboID
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-mKate2-P2A-TurboID-NS was a gift from Rob Parton (Addgene plasmid # 166568 ; http://n2t.net/addgene:166568 ; RRID:Addgene_166568) -
For your References section:
In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish. Xiong Z, Lo HP, McMahon KA, Martel N, Jones A, Hill MM, Parton RG, Hall TE. Elife. 2021 Feb 16;10. pii: 64631. doi: 10.7554/eLife.64631. 10.7554/eLife.64631 PubMed 33591275