T-TRIM33-4
(Plasmid
#166550)
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PurposescFv of a human scaffold targeting E3 ubiquitin-protein ligase TRIM33. Antigen coverage aa 882-1127 of 1127
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAT6
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Backbone manufacturerDDD, Science for Life Laboratory
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Vector typeBacterial Expression ; Recombinant Antibody Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesingle chain-Fv, scFv
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SpeciesH. sapiens (human)
- Promoter LacZ
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Tags
/ Fusion Proteins
- OmpA leader sequence (N terminal on insert)
- 3xFLAG, His6 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Target NCBI gene ID: 51592
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T-TRIM33-4 was a gift from Susanne Gräslund (Addgene plasmid # 166550 ; http://n2t.net/addgene:166550 ; RRID:Addgene_166550)