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Addgene

O-AARS-32
(Plasmid #166540)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166540 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAT6
  • Backbone manufacturer
    DDD, Science for Life Laboratory
  • Vector type
    Bacterial Expression ; Recombinant Antibody Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    single chain-Fv, scFv
  • Species
    H. sapiens (human)
  • Promoter LacZ
  • Tags / Fusion Proteins
    • OmpA leader sequence (N terminal on insert)
    • 3xFLAG, His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genbank acc. no of scFv: MT074297. Target NCBI gene ID: 16. The antigen-containing plasmid can be found under Addgene catalog ID: 153047 (https://www.addgene.org/153047/)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    O-AARS-32 was a gift from Susanne Gräslund (Addgene plasmid # 166540 ; http://n2t.net/addgene:166540 ; RRID:Addgene_166540)

  • For your References section:

    Generation and validation of recombinant antibodies to study human aminoacyl-tRNA synthetases. Preger C, Wigren E, Ossipova E, Marks C, Lengqvist J, Hofstrom C, Andersson O, Jakobsson PJ, Graslund S, Persson H. J Biol Chem. 2020 Oct 9;295(41):13981-13993. doi: 10.1074/jbc.RA120.012893. Epub 2020 Aug 14. 10.1074/jbc.RA120.012893 PubMed 32817337
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