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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBI-EGFP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5200
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Vector typeMammalian Expression
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCyclin E
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
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Entrez GeneCCNE1 (a.k.a. CCNE, pCCNE1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A fragment of human cyclin E cDNA including the entire ORF was cloned into pBI-EGFP.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cyclin E-pBI-EGFP was a gift from Bert Vogelstein (Addgene plasmid # 16654 ; http://n2t.net/addgene:16654 ; RRID:Addgene_16654) -
For your References section:
Inactivation of hCDC4 can cause chromosomal instability. Rajagopalan H, Jallepalli PV, Rago C, Velculescu VE, Kinzler KW, Vogelstein B, Lengauer C. Nature. 2004 Mar 4. 428(6978):77-81. 10.1038/nature02313 PubMed 14999283
Map uploaded by the depositor.
