pCMV-Myc CDC4 R465C
(Plasmid #16653)

• Depositing Lab: Bert Vogelstein
• Publication: Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Backbone

• Vector backbone: pCMV-Myc
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDC4 R465C
• Alt name: Archipelago
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: R465C
• Entrez Gene: FBXW7 (a.k.a. AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: EBV rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A 1.5kb fragment of human CDC4 cDNA (nucleotides 374-1874), which encompasses the entire ORF, was cloned into pCMV-Myc.

Addgene's quality control sequence suggests there are some unexpected mutations in the gene. The effect these mutations have on function is unknown, but this is the plasmid used in the Vogelstein article, so it should behave as reported.