pCDNA5_FRT_TO_TurboID-mCherry-Cry2
(Plasmid
#166504)
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PurposeExpression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166504 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
- Backbone size w/o insert (bp) 5115
- Total vector size (bp) 8403
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- TurboID (N terminal on insert)
- mCherry-CRY2 (C terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA5_FRT_TO_TurboID-mCherry-Cry2 was a gift from Angelos Constantinou (Addgene plasmid # 166504 ; http://n2t.net/addgene:166504 ; RRID:Addgene_166504) -
For your References section:
TopBP1 assembles nuclear condensates to switch on ATR signaling. Frattini C, Promonet A, Alghoul E, Vidal-Eychenie S, Lamarque M, Blanchard MP, Urbach S, Basbous J, Constantinou A. Mol Cell. 2021 Jan 16. pii: S1097-2765(20)30991-6. doi: 10.1016/j.molcel.2020.12.049. 10.1016/j.molcel.2020.12.049 PubMed 33503405
Map uploaded by the depositor.
