pAB_PalkB_sfgfp_KanR
(Plasmid
#166503)
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PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSEVA231
- Total vector size (bp) 3698
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Modifications to backboneorigin of replication replaced with pBR322/rop (non-SEVA)
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesSynthetic
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Insert Size (bp)819
- Promoter PalkB
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GTTCTGAGGTCATTACTGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAB_PalkB_sfgfp_KanR was a gift from Sven Panke (Addgene plasmid # 166503 ; http://n2t.net/addgene:166503 ; RRID:Addgene_166503) -
For your References section:
Directed evolution of biofuel-responsive biosensors for automated optimization of branched-chain alcohol biosynthesis. Bahls MO, Platz L, Morgado G, Schmidt GW, Panke S. Metab Eng. 2021 Nov 9. pii: S1096-7176(21)00171-3. doi: 10.1016/j.ymben.2021.10.014. 10.1016/j.ymben.2021.10.014 PubMed 34767976
Map uploaded by the depositor.
