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Addgene

pCK01_PalkS_alkS
(Plasmid #166502)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166502 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCK01
  • Total vector size (bp) 6839
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    alkS
  • Species
    Pseudomonas putida GPo1
  • Insert Size (bp)
    2759
  • Mutation
    L401G
  • Promoter PalkS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCK01_PalkS_alkS was a gift from Sven Panke (Addgene plasmid # 166502 ; http://n2t.net/addgene:166502 ; RRID:Addgene_166502)
  • For your References section:

    Directed evolution of biofuel-responsive biosensors for automated optimization of branched-chain alcohol biosynthesis. Bahls MO, Platz L, Morgado G, Schmidt GW, Panke S. Metab Eng. 2021 Nov 9. pii: S1096-7176(21)00171-3. doi: 10.1016/j.ymben.2021.10.014. 10.1016/j.ymben.2021.10.014 PubMed 34767976