Lenti-CRISPR-V2-gYap-2
(Plasmid
#166482)
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PurposeExpresses spCas9 and a gRNA targeting mouse Yap
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLenti-CRISPR-V2
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegRNA for mouse Yap
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gRNA/shRNA sequenceUnspecified
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SpeciesM. musculus (mouse)
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Entrez GeneYap1 (a.k.a. Yap, Yap65, Yki, Yorkie)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-CRISPR-V2-gYap-2 was a gift from Shuguo Sun (Addgene plasmid # 166482 ; http://n2t.net/addgene:166482 ; RRID:Addgene_166482) -
For your References section:
Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy by promoting YAP phase separation. Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, Zhang L, Li Y, Yang XP, Sun S. Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 10.1016/j.molcel.2021.01.010. 10.1016/j.molcel.2021.01.010 PubMed 33606996