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Addgene

DNMT1 KO Hyg
(Plasmid #16648)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSL301
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3264
  • Vector type
    Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNMT1 knock-out construct
  • Alt name
    DNMT1
  • Species
    H. sapiens (human)
  • Entrez Gene
    DNMT1 (a.k.a. ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' arm is 4.3 kb. 3' arm is 3.0 kb. See author's map for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DNMT1 KO Hyg was a gift from Bert Vogelstein (Addgene plasmid # 16648 ; http://n2t.net/addgene:16648 ; RRID:Addgene_16648)

  • For your References section:

    DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Rhee I, Bachman KE, Park BH, Jair KW, Yen RW, Schuebel KE, Cui H, Feinberg AP, Lengauer C, Kinzler KW, Baylin SB, Vogelstein B. Nature. 2002 Apr 4. 416(6880):552-6. 10.1038/416552a PubMed 11932749
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