pUC19-YAP-mEGFP
(Plasmid
#166472)
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PurposeHDR template for tagging of endogenous mouse YAP N-terminus with mEGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYAP-mEGFP HDR template(mouse Yap)
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SpeciesM. musculus (mouse)
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Entrez GeneYap1 (a.k.a. Yap, Yap65, Yki, Yorkie)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19-YAP-mEGFP was a gift from Shuguo Sun (Addgene plasmid # 166472 ; http://n2t.net/addgene:166472 ; RRID:Addgene_166472) -
For your References section:
Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy by promoting YAP phase separation. Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, Zhang L, Li Y, Yang XP, Sun S. Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 10.1016/j.molcel.2021.01.010. 10.1016/j.molcel.2021.01.010 PubMed 33606996