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Addgene

SMAD4 KO Neo
(Plasmid #16646)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad4 knock-out construct
  • Alt name
    Smad4
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' arm is 2.2 kb. 3' arm is 4.7 kb. See author's map for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SMAD4 KO Neo was a gift from Bert Vogelstein (Addgene plasmid # 16646 ; http://n2t.net/addgene:16646 ; RRID:Addgene_16646)

  • For your References section:

    DEC1 is a downstream target of TGF-beta with sequence-specific transcriptional repressor activities. Zawel L, Yu J, Torrance CJ, Markowitz S, Kinzler KW, Vogelstein B, Zhou S. Proc Natl Acad Sci U S A. 2002 Mar 5. 99(5):2848-53. 10.1073/pnas.261714999 PubMed 11880636
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