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Addgene

pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)
(Plasmid #166422)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15B
  • Backbone manufacturer
    Novagen Inc., Madison, WI
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6333
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plasmodium falciparum PRL
  • Species
    Plasmodium falciparum
  • Insert Size (bp)
    625
  • Mutation
    Deleted nucleotides coding for 4 C-terminal amino acids
  • Promoter AmpR, lac1
  • Tag / Fusion Protein
    • N-terminal His-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer T7 Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif) was a gift from Kalle Gehring (Addgene plasmid # 166422 ; http://n2t.net/addgene:166422 ; RRID:Addgene_166422)
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