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pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)
(Plasmid #166421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15B
  • Backbone manufacturer
    Novagen Inc., Madison, WI
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6192
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Naegleria fowleri PRL
  • Species
    Naegleria fowleri
  • Insert Size (bp)
    484
  • Mutation
    Deleted nucleotides coding for 4 C-terminal amino acids
  • Promoter AmpR, lac1
  • Tag / Fusion Protein
    • N-terminal His-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer T7 Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-15b(+) Naegleria fowleri PRL (Δprenylation motif) was a gift from Kalle Gehring (Addgene plasmid # 166421 ; http://n2t.net/addgene:166421 ; RRID:Addgene_166421)

  • For your References section:

    Burst kinetics and CNNM binding are evolutionarily conserved properties of phosphatases of regenerating liver. Fakih R, Goldstein RH, Kozlov G, Gehring K. J Biol Chem. 2023 Apr;299(4):103055. doi: 10.1016/j.jbc.2023.103055. Epub 2023 Feb 22. 10.1016/j.jbc.2023.103055 PubMed 36822330
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