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pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast
(Plasmid #166412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166412 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE2-EF1a
  • Backbone size w/o insert (bp) 8069
  • Total vector size (bp) 9968
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hPOT1 K90E
  • Alt name
    CMM10, GLM9, HPOT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1899
  • Mutation
    K90E
  • Entrez Gene
    POT1 (a.k.a. CMM10, CRMCC3, GLM9, HPOT1, PFBMFT8, TPDS3)
  • Promoter EF-1a
  • Tag / Fusion Protein
    • MYC (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NOT1 (unknown if destroyed)
  • 3′ cloning site PAC1 (unknown if destroyed)
  • 5′ sequencing primer GAACAAAAGTTGATTTCTGAAGAAGATTTG
  • 3′ sequencing primer TTAGATTACATCTTCTGCAACTGTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast was a gift from Agnel Sfeir (Addgene plasmid # 166412 ; http://n2t.net/addgene:166412 ; RRID:Addgene_166412)

  • For your References section:

    Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore. Pinzaru AM, Kareh M, Lamm N, Lazzerini-Denchi E, Cesare AJ, Sfeir A. Genes Dev. 2020 Dec 1;34(23-24):1619-1636. doi: 10.1101/gad.337287.120. Epub 2020 Oct 29. 10.1101/gad.337287.120 PubMed 33122293
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