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PIK3CA - Exon9 E545K (pFastBac)
(Plasmid #16640)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16640 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac HT
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4856
  • Vector type
    Mammalian Expression ; Baculoviral
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PIK3CA E545K
  • Alt name
    Phosphatidylinositol 3-kinase
  • Alt name
    p110 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3100
  • Mutation
    E545K
  • Entrez Gene
    PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer polyhedrin forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A fragment of human PIK3CA cDNA including the entire ORF was cloned into the pFastBac HT vector. The cDNA fragment can be removed with BamHI and HindIII for subcloning into other vectors.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PIK3CA - Exon9 E545K (pFastBac) was a gift from Bert Vogelstein (Addgene plasmid # 16640 ; http://n2t.net/addgene:16640 ; RRID:Addgene_16640)

  • For your References section:

    High frequency of mutations of the PIK3CA gene in human cancers. Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler KW, Vogelstein B, Velculescu VE. Science. 2004 Apr 23. 304(5670):554. 10.1126/science.1096502 PubMed 15016963
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