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pCI-Neo-PTPRT (R632X)
(Plasmid #16629)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16629 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PTPRT R632X
  • Alt name
    protein tyrosine phosphatase, receptor type, T
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • Mutation
    truncated R632X mutant
  • Entrez Gene
    PTPRT (a.k.a. RPTPrho)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 6kb fragment of PTPRT including the entire ORF and the 3'UTR was excised from KIAA clone (ha06139s1) with SgrAI and ScaI and cloned into the XmaI/EcoRV sites of pIRES2hyg2. A 1.9kb fragment containing PTPRT was digested with NheI and NotI and cloned into the pCI-Neo expression vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo-PTPRT (R632X) was a gift from Bert Vogelstein (Addgene plasmid # 16629 ; http://n2t.net/addgene:16629 ; RRID:Addgene_16629)

  • For your References section:

    Mutational analysis of the tyrosine phosphatome in colorectal cancers. Wang Z, Shen D, Parsons DW, Bardelli A, Sager J, Szabo S, Ptak J, Silliman N, Peters BA, van der Heijden MS, Parmigiani G, Yan H, Wang TL, Riggins G, Powell SM, Willson JK, Markowitz S, Kinzler KW, Vogelstein B, Velculescu VE. Science. 2004 May 21. 304(5674):1164-6. 10.1126/science.1096096 PubMed 15155950
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