pCAR-OF reporter
(Plasmid #16627)

• Depositing Lab: Bert Vogelstein
• Publication: Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10400
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CAR-OF
• Alt name: CAR out-of-frame
• Tag / Fusion Protein:
  • beta-galactosidase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: LacZ-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The reporter construct pCAR-OF (out of frame) contains a hygromycin resistance gene plus a beta-galactosidase gene containing a 58-bp out-of-frame poly(C-A) tract at the 5' end of its coding region.

The pCAR-OF reporter will not generate beta-galactosidase activity unless a frame-restoring mutation (i.e., insertion or deletion) arises following transfection.