pBI-GFP-Bub1-S492Y
(Plasmid #16625)

• Depositing Lab: Bert Vogelstein
• Publication: Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Backbone

• Vector backbone: pBI-GFP
• Backbone manufacturer: Vogelstein Lab
• Backbone size w/o insert (bp): 5099
• Vector type: Mammalian Expression
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BUB1 S492Y
• Alt name: Bub1
• Species: H. sapiens (human)
• Mutation: S492Y
• Entrez Gene: BUB1 (a.k.a. BUB1A, BUB1L, hBUB1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: EBV rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBI-GFP-Bub1 S492Y was constructed by cloning RT-PCR products from tumour line V429 into the NotI and SalI sites of pBI-GFP.