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Addgene

pcDNA3.1-rsAKARev(T/A)
(Plasmid #166232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166232 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5399
  • Total vector size (bp) 7676
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
  • Alt name
    rsAKARev(T/A)
  • Insert Size (bp)
    2277
  • Mutation
    changed Threonine 506 in rsAKARev to Alanine
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer TAGAAGGCACAGTCGAGGCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pre-print of this article is available on BioRxiv.org
doi: https://doi.org/10.1101/2021.01.06.425528

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-rsAKARev(T/A) was a gift from Peter Dedecker (Addgene plasmid # 166232 ; http://n2t.net/addgene:166232 ; RRID:Addgene_166232)

  • For your References section:

    Simultaneous readout of multiple FRET pairs using photochromism. Roebroek T, Vandenberg W, Sipieter F, Hugelier S, Stove C, Zhang J, Dedecker P. Nat Commun. 2021 Mar 31;12(1):2005. doi: 10.1038/s41467-021-22043-0. 10.1038/s41467-021-22043-0 PubMed 33790271
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