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Addgene

pLV-CS-154
(Plasmid #166230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVSIN-CMV-Pur
  • Backbone manufacturer
    TaKaRa
  • Backbone size w/o insert (bp) 5819
  • Total vector size (bp) 12971
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    U6-gRNA EGFP targetting
  • Species
    Synthetic
  • Promoter Human U6

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    nCas9-PmCDA1-UGI-2A-mCherry
  • Species
    Synthetic
  • Promoter CMV promoter

Cloning Information for Gene/Insert 2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-CS-154 was a gift from Nozomu Yachie (Addgene plasmid # 166230 ; http://n2t.net/addgene:166230 ; RRID:Addgene_166230)

  • For your References section:

    CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations. Fukushima T, Tanaka Y, Adachi K, Masuyama N, Tsuchiya A, Asada S, Ishiguro S, Mori H, Seki M, Yachie N, Goyama S, Kitamura T. Sci Rep. 2021 Dec 13;11(1):23889. doi: 10.1038/s41598-021-02986-6. 10.1038/s41598-021-02986-6 PubMed 34903756
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