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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBI-GFP
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Backbone manufacturerVogelstein Lab
- Backbone size (bp) 5099
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Vector typeMammalian Expression
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expression vector pBI-GFP was constructed by insertion of the blunt-ended HindIII/NotI GFP fragment of phGFP-S65T (Clontech) into the EcoRV site of pBI (Clontech).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBI-GFP was a gift from Bert Vogelstein (Addgene plasmid # 16623 ; http://n2t.net/addgene:16623 ; RRID:Addgene_16623) -
For your References section:
Mutations of mitotic checkpoint genes in human cancers. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B. Nature. 1998 Mar 19. 392(6673):300-3. 10.1038/32688 PubMed 9521327
Map uploaded by the depositor.
