pBI-GFP
(Plasmid #16623)

• Purpose: (Empty Backbone)
• Depositing Lab: Bert Vogelstein
• Publication: Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Backbone

• Vector backbone: pBI-GFP
• Backbone manufacturer: Vogelstein Lab
• Backbone size (bp): 5099
• Vector type: Mammalian Expression
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: EBV rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression vector pBI-GFP was constructed by insertion of the blunt-ended HindIII/NotI GFP fragment of phGFP-S65T (Clontech) into the EcoRV site of pBI (Clontech).