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Addgene

GRK2-CAAX
(Plasmid #166224)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166224 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression
  • Selectable markers
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bovine GRK2-CAAX
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    2070
  • Mutation
    adding CAAX sequence at C terminal
  • GenBank ID
    NM_174710 NM_174710
  • Entrez Gene
    GRK2 (a.k.a. ADRBK1)
  • Promoter CMV
  • Tag / Fusion Protein
    • no

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GRK2-CAAX was a gift from Robert Lefkowitz (Addgene plasmid # 166224 ; http://n2t.net/addgene:166224 ; RRID:Addgene_166224)

  • For your References section:

    Isoprenylation in regulation of signal transduction by G-protein-coupled receptor kinases. Inglese J, Koch WJ, Caron MG, Lefkowitz RJ. Nature. 1992 Sep 10;359(6391):147-50. doi: 10.1038/359147a0. 10.1038/359147a0 PubMed 1522899
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