PPARdelta KO vector
(Plasmid #16619)

• Depositing Lab: Bert Vogelstein
• Publication: Park et al Proc Natl Acad Sci U S A. 2001 Feb 27. 98(5):2598-603.

Backbone

• Vector backbone: pBluescript II KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPAR delta
• Species: H. sapiens (human)
• Entrez Gene: PPARD (a.k.a. FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map for more information. PKO2: used to delete exon 4 of PPAR delta. 5' arm is 1.8 kb and 3' arm is 4.0 kb.