IRES-puro-GFP
(Plasmid #16616)

• Depositing Lab: Bert Vogelstein
• Publication: Torrance et al Nat Biotechnol. 2001 Oct . 19(10):940-5.

Backbone

• Vector backbone: pIRESpuro2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Alt name: Green fluorescent protein
• Insert Size (bp): 700

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is an improved version of the vector used in the publication.

Note that although the SnapGene software recognizes the fluorescent protein as EYFP, this variant does not have the mutations required to convert GFP to YFP, and so should function more similarly to GFP.