Skip to main content
Addgene

pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP
(Plasmid #166150)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDEST17
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21-CodonPlus(DE3)-RIPL
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX5ΔPrD(1-535)
  • Species
    N. furzeri
  • Mutation
    ΔPrD(1-535)
  • Promoter T7
  • Tags / Fusion Proteins
    • His10-Smt3 (N terminal on backbone)
    • SNAP (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP was a gift from Daniel Jarosz (Addgene plasmid # 166150 ; http://n2t.net/addgene:166150 ; RRID:Addgene_166150)

  • For your References section:

    Identification of protein aggregates in the aging vertebrate brain with prion-like and phase separation properties. Itamar Harel , Yiwen R. Chen, Inbal Ziv, Param Priya Singh, Paloma Navarro Negredo, Uri Goshtchevsky, Wei Wang, Gwendoline Astre, Eitan Moses, Andrew McKay, Ben E. Machado, Katja Hebestreit, Sifei Yin, View Alejandro Sánchez Alvarado, Daniel F. Jarosz, Anne Brunet. BioRxiv 10.1101/2022.02.26.482115
Related items: