pAG426GAL-EGFP
(Plasmid
#166143)
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PurposeEGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS426
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic
- Promoter GAL1
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional Article: https://www.biorxiv.org/content/10.1101/2022.02.26.482120v1
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG426GAL-EGFP was a gift from Daniel Jarosz (Addgene plasmid # 166143 ; http://n2t.net/addgene:166143 ; RRID:Addgene_166143) -
For your References section:
Identification of protein aggregates in the aging vertebrate brain with prion-like and phase separation properties. Itamar Harel , Yiwen R. Chen, Inbal Ziv, Param Priya Singh, Paloma Navarro Negredo, Uri Goshtchevsky, Wei Wang, Gwendoline Astre, Eitan Moses, Andrew McKay, Ben E. Machado, Katja Hebestreit, Sifei Yin, View Alejandro Sánchez Alvarado, Daniel F. Jarosz, Anne Brunet. BioRxiv 10.1101/2022.02.26.482115
