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Addgene

FDXR KO-2A
(Plasmid #16614)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFredA
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 3838
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FDXR knockout
  • Alt name
    FDXR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • Mutation
    5' targeting element
  • Entrez Gene
    FDXR (a.k.a. ADR, ADXR, ANOA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

4.2 kb 5' targeting element (up to +1527). See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FDXR KO-2A was a gift from Bert Vogelstein (Addgene plasmid # 16614 ; http://n2t.net/addgene:16614 ; RRID:Addgene_16614)

  • For your References section:

    Ferredoxin reductase affects p53-dependent, 5-fluorouracil-induced apoptosis in colorectal cancer cells. Hwang PM, Bunz F, Yu J, Rago C, Chan TA, Murphy MP, Kelso GF, Smith RA, Kinzler KW, Vogelstein B. Nat Med. 2001 Oct . 7(10):1111-7. 10.1038/nm1001-1111 PubMed 11590433
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